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中华肥胖与代谢病电子杂志 ›› 2019, Vol. 05 ›› Issue (03) : 128 -136. doi: 10.3877/cma.j.issn.2095-9605.2019.03.002

所属专题: 文献

论著

肥胖大鼠袖状胃切除术后肠源性脂多糖的变化及其与体重变化的相关性研究
黎熊1, 刘斌1, 李佳俊1, 刘伟1,()   
  1. 1. 610000 成都,成都医学院第二附属医院?核工业四一六医院普外科
  • 收稿日期:2019-03-03 出版日期:2019-08-30
  • 通信作者: 刘伟
  • 基金资助:
    四川省卫计委科研项目基金(18PJ489)

Change of enterogenous lipopolysaccharide and its correlation analysis with weight change after sleeve gastrectomy in obese rats

Xiong Li1, Bin Liu1, Jiajun Li1, Wei Liu1,()   

  1. 1. Department of General Surgery, the Second Affiliated Hospital of Chengdu Medical College, Nuclear industry 416 hospital, Chengdu 610000, China
  • Received:2019-03-03 Published:2019-08-30
  • Corresponding author: Wei Liu
  • About author:
    Corresponding author: Liu Wei, Email:
引用本文:

黎熊, 刘斌, 李佳俊, 刘伟. 肥胖大鼠袖状胃切除术后肠源性脂多糖的变化及其与体重变化的相关性研究[J]. 中华肥胖与代谢病电子杂志, 2019, 05(03): 128-136.

Xiong Li, Bin Liu, Jiajun Li, Wei Liu. Change of enterogenous lipopolysaccharide and its correlation analysis with weight change after sleeve gastrectomy in obese rats[J]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2019, 05(03): 128-136.

目的

通过检测肥胖大鼠在袖状胃切除术前后体内脂多糖的变化及分布探究脂多糖与大鼠术后体重变化的相关性。

方法

(1)建立肥胖大鼠模型成功后测量大鼠体重、体长、糖耐量及胰岛素抵抗水平并随机分组。手术组行袖状胃切除术,术后每周随机处死5只大鼠。假手术组行剖腹探查术,处死及分组同手术组。(2)术后每2日测量两组大鼠体重。(3)取两组大鼠术前及术后粪便行革兰染色,计算菌群比例。(4)手术前后定时抽取两组大鼠尾静脉血液,检测其血浆LPS水平。(5)处死大鼠时,取两组大鼠肝脏、肠系膜、肾周脂肪、附睾脂肪等组织检测其LPS水平。(6)术后对各组大鼠行糖耐量试验及胰岛素抵抗水平检测。

结果

(1)术后手术组大鼠体重进行性下降,而假手术组大鼠体重下降后回升。(2)与假手术组相比,手术组大鼠术后粪便革兰阳性菌数量增多,革兰阴性菌减少。(3)术后手术组大鼠血清LPS水平下降,而假手术组大鼠血清LPS水平下降后回升并超过术前水平。且两组大鼠血清LPS水平与取血当时的体重水平有显著的直线相关性(P=0.0004)。(4)与假手术组相比,手术组大鼠腹腔脂肪中LPS水平随术后时间降低。(5)术后手术组大鼠糖耐量水平及HOMA-IR指数降低,而假手术组升高。

结论

(1)肥胖大鼠袖状胃切除术后,大鼠体内LPS水平的变化与其体重变化存在显著相关性;(2)肥胖大鼠袖状胃切除术后,大鼠糖耐量及胰岛素敏感性得到改善。

Objective

This study aims to explore the correlation by detecting the metabolic changes and internal distribution of the LPS contained in rat during preoperative and postoperative period of sleeve gastrectomy.

Methods

(1) After successful obese rats modeling did, the weight, body length, glucose tolerance and insulin resistance level were measured and grouping them randomized. Rats in surgical group were performed sleeve gastrectomy. Then execute 5 rats per week. Rats in sham operation group were performed exploratory Laparotomy, whose grouping and execution coincides with surgical group. (2) Rats weight of surgical group and sham operation group were measured once for two days after operation. (3) Faeces of rats from two groups were selected both preoperatively and postoperatively, which were used to analysis the proportion of the gram-positive bacterium and gram-negative bacterium. (4) Rats' blood in two groups were collected regularly both before and post operation to measure serum levels of LPS. (5) In surgical group and sham operation group, tissues of liver, mesentery fat, perirenal fat and epididymal fat were picked and grinded for tissue homogenate when rats were executed to quantify the LPS contained in these tissues. 6) Glucose tolerance tests and insulin resistance level tests were performed in each group both preoperatively and postoperatively.

Results

(1) The weight of rats in sham operation group rebound gradually after decreased, even more heavier than preoperative. Oppositely, the weight of rats in surgical group decreased progressively and then stabilized. (2) the proportion of gram-positive bacteria of surgical group were increased compared with sham operation group. On the contrary, the proportion of gram-negative bacteria of surgical group were reduced. (3) The rats' serum level of LPS in operation group declined obviously after operation, however, the rats' serum level of LPS in sham operation group climbed up higher than preoperative levels. Also, serum levels of LPS in the two groups have significant linear correlation with contemporaneous body weight (P=0.0004). (4) The LPS of operation group in abdominal fat significantly decreased compared with sham operation group. (5) The glucose tolerance levels and HOMA IR of rats in surgical group significantly decreased after operation, while these index in sham operation group obviously elevated.

Conclusions

(1) There is a close relationship between their LPS and body weight after sleeve gastrectomy of obese rats; (2) After sleeve gastrectomy of obese rats, glucose tolerance and insulin sensitivity are improved.

表1 术前大鼠体重、体长、糖耐量水平及胰岛素抵抗水平
图1 高脂饲料喂养组大鼠(左)与普通饲料喂养组大鼠(右)外观体型比较
图2 手术组大鼠与假手术组大鼠体重变化曲线
表2 术前及术后第4周手术组与假手术组大鼠粪便菌群比例变化
图3 术前及术后4周粪便革兰染色油镜下随机视野。3A:手术组大鼠术前粪便革兰染色;3B:假手术组大鼠术前粪便革兰染色;3C:手术组术大鼠术后4周粪便革兰染色;3D:假手术组大鼠术后4周粪便革兰染色
表3 手术组及假手术组大鼠各期血清LPS水平
图4 手术组及假手术组大鼠各期血清LPS水平
图5 大鼠血清LPS水平与体重直线回归关系
图6 手术组及假手术组大鼠各期组织LPS水平
表4 手术组、假手术组大鼠术前及术后第28天糖耐量水平及胰岛素抵抗水平
图7 袖状胃切除范围及术后4周残胃情况
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