建立饮食诱导的肥胖型（DIO）小鼠袖状胃切除术（SG）与改良Roux-en-Y胃旁路术（RYGB）模型，为代谢外科治疗肥胖伴2型糖尿病（T2DM）机制研究提供动物模型。

选取体质量（BMI）、周龄等均无明显差异的13周育龄雄性DIO小鼠，小鼠平均体质量（33.69±2.25）g，平均空腹血糖（11.66±1.52）mmol/L。显微镜下成功建立小鼠SG组（n=7）、改良RYGB组（n=10）和对照组（n=6）模型。术前及术后第l、2、4、7、14天进行小鼠体重和空腹血糖测量。

SG组小鼠存活率为85.71%（6/7），改良RYGB组存活率为60.00%（6/10）。术后SG组和改良RYGB组小鼠体重进行性下降，而对照组小鼠体重变化不明显。术后第4、7、14天，SG组和改良RYGB组体重水平较对照组显著下降（P<0.05）。两种手术组小鼠术后不同观察时间段空腹血糖水平较对照组有明显的差异性（P<0.05）。

本研究成功建立DIO小鼠显微镜下袖状胃切除术与改良Roux-en-Y胃旁路术模型。通过DIO小鼠建立改良Roux-en-Y胃旁路术和袖状胃切除术小鼠模型是有效和可行的。此模型为代谢外科治疗肥胖伴T2DM机制的研究提供一个更为合理的动物模型。

To establish sleeve gastrectomy (SG) and modified Roux-en-Y gastric bypass (RYGB) model in diet-induced obese (DIO) mice and applying to basic mechanical research on mechanisms of metabolic surgery.

Weight and age matched 5-week-old male C57 BL/6 mice were selected and fed with 60% high fat diet for 8 weeks for induction of type 2 diabetes mellitus. The average weight and fasting plasma glucose levels were (33.79±2.0) g and (11.5±0.8) mmol/L respectively. Mice were randomized into modified SG group (n=7), RYGB group (n=10) and control group (n=6). The first two operations were performed under microscope. Traditional operation techniques were applied for the SG group while the modified RYGB group was treated with lower esophageal ligation and esophago-intestinal anastomosis. Body weight and fasting blood glucose levels were measured pre- and post-operative day (POD) 1, 2, 4, 7 and 14.

The general survival rate of SG group was 85.71% (6/7) and 60% (6/10) in modified RYGB group. SG group and modified RYGB group showed significantly more weight loss and blood glucose reduction than that in control group. SG and modified RYGB groups presented significantly lower body masses at POD 4, 7 and 14 (P<0.05). The fasting blood glucose in the SG group and modified RYGB group was significantly lower than that in the control group (P<0.05).

SG and modified RYGB bypass models were successfully established in DIO mice. Modified RYGB bypass and SG in DIO mice were effective and feasible. They could also provide a more reasonable model for the basic research and better understanding of mechanism of metabolic surgery in those suffered from obesity with type 2 diabetes mellitus patients.