To explore the classification and structural characteristics of intestinal flora in obese mice induced by high-fat diet.

Eight week old SPF male kunming mice were randomly divided into two groups: normal group (NC group, n=8) and obese group (Obs group, n=8).The obese mice (Obs) model was induced by high-fat diet. The intestinal microflora of mice was detected by 16s rRNA high-throughput sequencing technology, and the differences between high-fat diet mice and normal mice were analyzed and compared.

The effective sequence of mouse fecal flora in Obs group was clustered into 261 operation units (OTUs), which was lower than that in NC group (353 OTUs); The ACE and Chaol indexes of Obs group were lower than those of NC group, and the Simpson index of Obs group was higher than that of NC group, indicating that the flora richness and diversity of Obs group were lower than those of NC group. The contents of Bacteroidales, Campylobacteres, Lactobacillus and Enterobacteriaceae in the bacteria genus of Obs group werelower than those of NC group (P<0.05).The contents of Desulfovifrionales and Erysipelothrichales in the fecal flora of Obs mice were higher than those in the normal control group (P<0.05).

The diversity of intestinal microflora in obesity induced mice decreased significantly, and the distribution of intestinal microflora changed significantly.