Effects of miR-1908 on cell proliferation, cell cycle, apoptosis of human preadipocytes-visceral

doi:10.3877/cma.j.issn.2095-9605.2019.02.003

Abstract

Abstract:

Objective

To study the effect of miR-1908 on cell proliferation,cell cycle,apoptosis of human preadipocytes-visceral.

Methods

human preadipocytes-visceral were cultured and divided into three treatment groups—inhibitor group (transfected with chemically modified miR-1908-5p inhibitor), empty vector group (transfected empty carrier) and control group (without any treatment).CCK8 assay was used to detect the proliferation of HPA-v, Tunnel method was used to detect the apoptosis, Flow cytometry was used to detect cell cycle.

Results

CCK8 assay showed that the absorbance values of miR-1908 inhibitor group (0.61±0.07) was significantly lower than that of the control group (1.01±0.05) (P<0.05). Tunel results showed that the apoptotic rate of miR-1908 inhibitor group (0.27±0.03)% was significantly higher than that of control group (0.13±0.05)% (P<0.05). The results of flow cytometry showed that miR-1908 inhibitor might induce the G0/G1 phase cell cycle arrest.

Conclusions

Inhibition of miR-1908 expression could significantly inhibit HPA-v cell proliferation, induced apoptosis and change the cell cycle distribution.

Key words: MiR-1908, Adipocytes, Proliferation, Apoptosis, Cell cycle

Bingbing Guo, Jinyan Fu, Jingjing Pei, Xinye Jiang. Effects of miR-1908 on cell proliferation, cell cycle, apoptosis of human preadipocytes-visceral[J]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2019, 05(02): 79-84.

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References 22

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