miR-1908对人内脏前体脂肪细胞增殖、细胞凋亡和细胞周期的影响

doi:10.3877/cma.j.issn.2095-9605.2019.02.003

中华肥胖与代谢病电子杂志 ›› 2019, Vol. 05 ›› Issue (02) : 79 -84. doi: 10.3877/cma.j.issn.2095-9605.2019.02.003

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论著

miR-1908对人内脏前体脂肪细胞增殖、细胞凋亡和细胞周期的影响

郭冰冰¹, 付锦艳¹, 裴晶晶¹, 蒋新液¹^,^†(

)

^1. 214002　无锡，南京医科大学附属无锡妇幼保健院

收稿日期:2019-04-01 出版日期:2019-05-30
通信作者: 蒋新液
基金资助:
无锡市"科教强卫工程"医学青年人才项目(QNRC091)

Effects of miR-1908 on cell proliferation, cell cycle, apoptosis of human preadipocytes-visceral

Bingbing Guo¹, Jinyan Fu¹, Jingjing Pei¹, Xinye Jiang¹^,^†()

^1. Department of the Children Health, The Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China

Received:2019-04-01 Published:2019-05-30
Corresponding author: Xinye Jiang
About author:
Corresponding author: Jiang Xinye, Email: fyjxy2110@163.com

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目的

研究miR-1908对人内脏前体脂肪细胞（HPA-v）增殖、细胞凋亡和细胞周期的影响。

方法

体外培养HPA-v脂肪细胞,并根据实验分为抑制剂组（转染经化学修饰的miR-1908-5p抑制剂）、空载体组（转染空载体）、对照组（未进行任何处理），CCK8（Cell Counting Kit-8）法用于检测细胞的增殖情况，流式细胞仪检测细胞周期、Tunnel法检测细胞凋亡情况。

结果

CCK8结果显示miR-1908抑制剂组的吸光度（OD）值（0.61±0.07）明显低于对照组（1.01±0.05）（P<0.05），抑制miR-1908具有抑制HPA-v脂肪细胞增殖的作用；Tunel结果表示miR1908抑制剂组凋亡率（0.27±0.03）%明显大于对照组（0.13±0.05）%（P<0.05）；流式细胞测定的结果为G0/G1期细胞增加（与对照组相比，P<0.05），表明抑制miR-1908表达可能通过诱导HPA-v脂肪细胞G0/G1期阻滞抑制HPA-v脂肪细胞增殖。

结论

下调miR-1908表达能明显抑制人内脏前体脂肪细胞增殖，诱导细胞凋亡及改变细胞周期分布。

关键词: miR-1908, 脂肪细胞, 增殖, 凋亡, 细胞周期

Objective

To study the effect of miR-1908 on cell proliferation,cell cycle,apoptosis of human preadipocytes-visceral.

Methods

human preadipocytes-visceral were cultured and divided into three treatment groups—inhibitor group (transfected with chemically modified miR-1908-5p inhibitor), empty vector group (transfected empty carrier) and control group (without any treatment).CCK8 assay was used to detect the proliferation of HPA-v, Tunnel method was used to detect the apoptosis, Flow cytometry was used to detect cell cycle.

Results

CCK8 assay showed that the absorbance values of miR-1908 inhibitor group (0.61±0.07) was significantly lower than that of the control group (1.01±0.05) (P<0.05). Tunel results showed that the apoptotic rate of miR-1908 inhibitor group (0.27±0.03)% was significantly higher than that of control group (0.13±0.05)% (P<0.05). The results of flow cytometry showed that miR-1908 inhibitor might induce the G0/G1 phase cell cycle arrest.

Conclusions

Inhibition of miR-1908 expression could significantly inhibit HPA-v cell proliferation, induced apoptosis and change the cell cycle distribution.

Key words: MiR-1908, Adipocytes, Proliferation, Apoptosis, Cell cycle

图1 细胞培养图

图2 转染miR-1908抑制剂后，CCK8法检测对HPA-v脂肪细胞增殖的影响

图3 转染miR-1908抑制剂后各组脂肪细胞凋亡观察图

图4 转染miR-1908抑制剂后HPA-v脂肪细胞凋亡结果分析柱状图

图5 转染miR-1908抑制剂后，流式细胞术检测各组HPA-v脂肪细胞周期情况

图6 流式细胞术检测miR-1908表达变化对各组脂肪细胞周期的影响

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