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中华肥胖与代谢病电子杂志 ›› 2025, Vol. 11 ›› Issue (01) : 53 -61. doi: 10.3877/cma.j.issn.2095-9605.2025.01.008

论著

体质量指数、2 型糖尿病、生活方式因素与胃食管反流病的关系:一项双样本孟德尔随机化研究
顾家宁1, 韩国达2, 闫昊然1, 李正旗3, 王亮2,()   
  1. 1. 061000 沧州,河北医科大学附属沧州市中心医院
    2. 061000 沧州,沧州市中心医院胃肠外一科
    3. 062550 任丘,华北油田采一社区卫生服务中心
  • 收稿日期:2024-10-15 出版日期:2025-02-28
  • 通信作者: 王亮
  • 基金资助:
    沧州市重点研发计划指导项目(172302017)

Association of BMI,type 2 diabetes,lifestyle factors with gastroesophageal reflux disease:A twosample Mendelian randomization study

Jianing Gu1, Guoda Han2, Haoran Yan1, Zhengqi Li3, Liang Wang2,()   

  1. 1. Cangzhou Central Hospital,Hebei Medical University,Cangzhou 061000,China
    2. Department of Gastrointestinal Surgery I,Cangzhou Central Hospital,Cangzhou 061000,China
    3. Caiyi Community Health Service Center of Huabei Oilfield,Renqiu 062550,China
  • Received:2024-10-15 Published:2025-02-28
  • Corresponding author: Liang Wang
引用本文:

顾家宁, 韩国达, 闫昊然, 李正旗, 王亮. 体质量指数、2 型糖尿病、生活方式因素与胃食管反流病的关系:一项双样本孟德尔随机化研究[J/OL]. 中华肥胖与代谢病电子杂志, 2025, 11(01): 53-61.

Jianing Gu, Guoda Han, Haoran Yan, Zhengqi Li, Liang Wang. Association of BMI,type 2 diabetes,lifestyle factors with gastroesophageal reflux disease:A twosample Mendelian randomization study[J/OL]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2025, 11(01): 53-61.

目的

探讨BMI、2型糖尿病、生活方式因素与胃食管反流病(GERD)之间潜在的因果关系。

方法

通过双样本孟德尔随机化(MR)从GWAS数据库中提取了与各暴露因素及GERD相关的单核苷酸多态性(SNPs),并筛选出合适的工具变量(IVs)。通过Ensemble网站查询IVs的相关表型数据,以排除与混杂因素相关的SNPs。本研究采用逆方差加权法(IVW)、加权中位数法(MW)、MR Egger法等五种统计方法进行数据分析,并进行了异质性检验、水平多效性检验以及敏感性分析。使用比值比(OR)和95%置信区间(CI)来评估BMI、2型糖尿病、生活方式因素与GERD之间的因果关联。

结果

BMI、2型糖尿病、吸烟、饮酒、咖啡摄入量和GERD之间存在正向因果关系:1.66(95%CI:1.39~1.97)、1.05(95%CI:1.02~1.09)、3.03(95%CI:2.36~3.89)、1.78(95%CI:1.57~2.03)、1.22(95%CI:1.03~1.44)。

结论

BMI、2型糖尿病、吸烟、饮酒、咖啡摄入量具有与GERD风险相关的遗传证据。

Objective

The purpose of this study was to investigate the potential causal relationship between gastroesophageal reflux disease (GERD) and BMI,type 2 diabetes,lifestyle factors.

Methods

We extracted single nucleotide polymorphisms (SNPs) associated with various exposure factors and GERD from the GWAS database by two-sample Mendelian randomization analysis (MR),and selected appropriate instrumental variables (IVs).The phenotypic data of IVs were searched through the Ensemble website to exclude SNPs associated with confounding factors.In this study,five statistical methods such as inverse variance weighting (IVW),weighted median (MW),MR Egger method were used for data analysis,and heterogeneity,level multi-effect test and sensitivity analysis were carried out.Odds ratio (OR) and 95 %confidence interval (CI) were used to assess the causal association between GERD and BMI,type 2 diabetes,lifestyle factors.

Results

There was a positive causal relationship between between GERD and BMI,type 2 diabetes,lifestyle factors:1.66 (95%CI:1.39~1.97),1.05 (95%CI:1.02~1.09),3.03 (95%CI:2.36~3.89),1.78(95%CI:1.57~2.03),1.22 (95%CI:1.03~1.44),respectively.

Conclusions

BMI,type 2 diabetes,smoking,drinking,and coffee intake had genetic evidence associated with GERD risk.

图1 工具变量筛选原理
表1 暴露和结局数据信息
图2 孟德尔随机化分析结果及汇总森林图
图3 两样本孟德尔随机化散点图。3A为BMI为暴露因素组;3B为2型糖尿病为暴露因素组;3C为吸烟为暴露因素组;3D为饮酒为暴露因素组;3E为咖啡摄入量为暴露因素组
图4 敏感性分析结果。4A为BMI为暴露因素组;4B为2型糖尿病为暴露因素组;4C为吸烟为暴露因素组;4D为饮酒为暴露因素组;4E为咖啡摄入量为暴露因素组
图5 两样本孟德尔随机化漏斗图。5A为BMI为暴露因素组;5B为2型糖尿病为暴露因素组;5C为吸烟为暴露因素组;5D为饮酒为暴露因素组;5E为咖啡摄入量为暴露因素组
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